eNUVIO的OMEGA系列微流體設(shè)備非常適合涉及神經(jīng)元共培養(yǎng)和分區(qū)的實(shí)驗(yàn)。該裝置的每個(gè)腔室由一系列微流體通道物理分離。微通道的小尺寸用于流體隔離每個(gè)相鄰的腔室,但足夠大以允許神經(jīng)突/軸突突出并與位于相鄰腔室中的培養(yǎng)物相互作用。每個(gè)OMEGA4設(shè)備都包含兩對(duì)相鄰的腔室,這意味著可以使用一個(gè)設(shè)備進(jìn)行兩個(gè)獨(dú)立的實(shí)驗(yàn)。
OMEGA的開放式腔室設(shè)計(jì)使科學(xué)家能夠直接訪問他們的共同培養(yǎng)物,使研究人員能夠探索標(biāo)準(zhǔn)封閉腔室設(shè)備無法實(shí)現(xiàn)的新型實(shí)驗(yàn)范式。例如,腔室足夠大以容納直徑為幾毫米的組織,可輕松容納組織外植體或自組裝的3D培養(yǎng)物(例如球體、類器官)。此外,傳統(tǒng)的膜片鉗電極可以公開訪問培養(yǎng)物,使研究人員能夠檢查培養(yǎng)物的電生理特性。
OMEGA設(shè)備發(fā)貨后即可使用。它們是無菌的、親水的,并且預(yù)先粘合到高透射率#1.5玻璃蓋玻片上。只需涂上生長室并鋪板您的細(xì)胞——就這么簡單。使用OMEGA設(shè)備可以更輕松地長時(shí)間保持健康的培養(yǎng)物,因?yàn)槊總€(gè)套件中都包含蒸發(fā)最小化插件。這些簡單的可重復(fù)使用的培養(yǎng)插件不僅包含一個(gè)內(nèi)置的圓形孔,可在充滿液體時(shí)降低設(shè)備腔室的蒸發(fā)率,它們還用于物理穩(wěn)定設(shè)備,以便在培養(yǎng)室內(nèi)安全運(yùn)輸設(shè)備。
? Neuromuscular junction (NMJ) in the context of ALS, Kennedy’s disease (SBMA), Duchenne’s muscular dystrophy
* e.g. motor neuron/muscle co-culture
? Nigrostriatal pathway in the context of Parkinson’s disease
* e.g. dopaminergic neuron (SNc)/GABAergic neuron (striatal) co-culture
? Sensory system in the context of nociception and mechanosensation
* e.g. skin fibroblast/DRG co-culture
? Myelination
* e.g. neuron/oligodendrocyte or Schwann cell co-culture
? Enteric nervous system
* e.g. enteroendocrine cells/vagal neuron co-culture
? iPSC-derived cultures
? Self-assembled 3D cultures (e.g. spheroids, organoids)
? Primary cultures
? Tissue explants
? Immortalized Cell lines
? Glass coverslip: 22mm, round, #1.5 thickness
? Number of microchannels per interface: 70
? Microchannel length: ~640µm
? 4 x OMEGA4 devices (each containing 2 interfaces)
? 4 x evaporation minimizing culture inserts (for 35mm dishes)
? 4 x sterile culture dishes (35mm, round)
? 1 x microscopy adapter for OMEGA series devices
? 4 x OMEGA4 devices (each containing 2 interfaces)
? 4 x sterile culture dishes (35mm, round)